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KMID : 0356420000180020105
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Journal of Korean Andrology
2000 Volume.18 No. 2 p.105 ~ p.110
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Analysis of Calcium-Binding Proteins of Mouse Spermatozoa by 2-Dimensional SDS-PAGE
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Gye Myung Chan
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Abstract
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Purpose: To verify the expression of calcium-binding proteins in mouse spermatozoa.
Materials and Methods: Mouse sperm proteins were subjected to 2-dimensional SDS-PAGE combined with calcium shift in the presence or absence of Ca2+, Zn2+ or EDTA.
Results: In the epididymal sperm extracts, 10 kinds of protein spots showed mobility shift in the gel containing Ca2+. Most of the CBPs disappeared after the acrosome reaction (AR)induced by Ca2+-ionophore A23187, suggesting that they originated from acrosome and/or the plasma membrane overlaying the acrosome.
Conclusion: The CBPs might be involved in acrosome reaction of mouse spermatozoa. Protein database of sperm CBPs might be useful for diagnosis of male infertility.
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KEYWORD
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Calcium-binding proteins, Spermatozoa, Mouse,
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